Pre-made lentivirus express GFP fluorescent reporter under a minimal promoter inserted with 6 tandem repeats of hypoxia transcriptional response element (H-RE), for detecting Hypoxia-Inducible Factors (HIF)-reregulated signaling pathway acitvity.
This lentivirus also contain the puromycin selection marker under the constitutive RSV promoter for antibiotic selection. See Product Manual for details (.pdf).
If desired, you can also use a pathway “Control Lentivirus” to establish the No-response control profile to your pathway stimulus. The corresponding pathway control lentivirus is: CAT#: Path-Ctr1.
This is concentrated lentivirus provided in PBS solution, used for hard-to-transduced cell types or for serum-free cell culture.
Amount: 200ul/per vial, at 1 x 108 IFU/ml in PBS.