Lentivirus expresses the Human betaherpesvirus 5 (HHV-5)’ Envelope Protein. It expresses the glycoprotein protein B (NCBI ID: AKI13965.1, click to see its Amino Acid sequence), of Human betaherpesvirus 5 (HHV-5), with a poly-histidine tag at the C-terminus (6-His tag) which allow the purification of the antigen via Ni NTA affinity column.
The target was expressed under enhanced CMV promoter. A RFP-Blasticidin fusion dual selection marker under RSV promoter, allows to sort or select transduced cells via RFP fluorescent signal or via Blasticidin antibiotic. RFP signal provides a convenient, realtime monitoring the lentivirus performance. See Product Manual for details (.pdf).
HHV-5 Glycoprotein B is expressed on the surface of the virus. It plays critical roles in the entry of virus into cells and fusion of infected cells. It can be used as the antigen for virus detection, diagnostic assays, antibody and vaccines development, and other assays.
Amount: 200ul, at 1 x 108 IFU/ml, concentrated lentivirus, provided in PBS