Pre-made Lentivirus expressing Human Telomerase Reverse Transcriptase (hTERT, NM_198253.2), under suCMV promoter. A RFP-Puromycin fusion dual marker was expressed under RSV promoter, which allows to select the transduced cell via Puromycin antibiotic or cell sorted by RFP fluorescent signal.
hTERT is widely used for cell immortalization, used alone, or together with other cell immortalization factors, like SV40 large T-antigen. See Product Manual (.pdf).
Amount: This is the concentrated lentivirus, provided in PBS solution as 200ul/per vial at 1 x 108 IFU/ml, best used for serum free cell culture, hard-to-infected cell types, or In Vivo application.