Pre-made Lentivirus expressing Human Telomerase Reverse Transcriptase (hTERT, NM_198253.2), under enhanced EF1a promoter. A GFP-Puromycin fusion dual marker was expressed under RSV promoter, which allows to select the transduced cell via Puromycin antibiotic, or via cell sorting by GFP fluorescent signal.
hTERT is widely used for cell immortalization, used alone or together with other cell immortalization factors, like SV40 large T-antigen. See Product Manual (.pdf).
Amount: 200ul/per vial at 1 x 107 IFU/ml