Transformed from Human ES-2 cells (human ovarian carcinoma), express tetracycline represser (TetR) gene. TetR was expressed under suCMV promoter. A Puromycin antibiotic marker was expressed under RSV promoter. The following expression cassette was inserted into cell line’s genome.
The TetR stable cell lines can be used together with inducible expression lentivirus for the inducible target expression or generation of the target specific inducible expression cell lines.
Completed culture medium:
McCoy’s 5a Medium / 10% FBS / Pen-Strep, 0.1 mM Non-Essential Amino Acids; optionally add: 0.5 ug/ml final Puromycin.
Sold at: 1 vial x (>2 x 106 cells)/vial, see Product Manual for details.