Human MDA-MB-468 / Luciferase & GFP Stable Cells

$4,000.00

human MDA-MB-468 cell line co-express Luciferase and GFP dual report, containing puromycin selection.

Description

Human MDA-MB-468 Cell line is derived from the pleural effusion of a 51-year-old black woman in 1977, with metastatic adenocarcinoma of the breast. It has proven useful for the study of metastasis, migration, and breast cancer proliferation, and used to test chemical breast cancer treatments

This Luciferase & GFP / human MDA-MB-468 cell line was transformed via Lentivirus transduction. It has the Luciferase and GFP was constitutively co-expressed under EF1a promoter, as two separate proteins (not as fusion), mediated via 2A element. The Puromycin resistance marker was expressed under Rsv promoter. The following express cassette was integrated into cell-line’s genome. 

GFP and Luciferase co-expression under EF1a promoter

Each cell demonstrates a strong GFP fluorescent signal (see sample image below). Its chem-luminescence signal was verified via luciferase assay using the D-luciferin substrate. And its signal can be detected, in vitro and in vivo.

GFP and luciferase dual reporter / MDA-MB-468 Cell line

Sold at:  1 vial x (2 x 106 cells)/vial, see details in Product Manual.

Cat# SC092-LG