Transformed from human MIA PaCa-2 cell line (a human pancreatic cancer cell line), the Luciferase and RFP was constitutively co-expressed under EF1a promoter as two separate protein (not as fusion), mediated via 2A element. The Puromycin resistance marker was expressed under Rsv promoter. The following express cassette was integrated into cell line’s genome.
Each cell demonstrates the strong GFP fluorescent signal (see sample image below), and Bio-luminescence signal via Luciferase substrate (D-luciferin), In Vivo, and In Vitro.
(Note: It is the pooled stable cell population, not originated from a single cell colony. Thus you will observe the uneven RFP signal intensity amount cells).
Sold at: 1 vial x (2 x 106 cells)/vial, see details in Product Manual.