Human U2OS / (Luciferase & GFP) Stable Cells



Human Bone Osteosarcoma Epithelial Cells (U2OS) is an established human epithelial cell line isolated back in the 1960s from a 15-year-old female suffering from bone cancer or osteosarcoma. U2OS cells exhibit epithelial adherent morphology. Cells are positive for IGF-I) and IGF II receptors and express a number of antigens, including blood type A, Rh+, HLA A2, Aw30, B12, Bw35, and B40(+/-). The U2OS cell line is extensively used as a circadian rhythm model, including in high-throughput screening studies.

Transformed from U2OS cell line, this cell line has the Luciferase and GFP constitutively co-expressed under EF1a promoter as two separate protein (not as fusion), mediated via 2A element. The Puromycin resistance marker was expressed under Rsv promoter. The following express cassette was integrated into cell-line’s genome. 


Each cell demonstrates the strong GFP fluorescent signal (see sample image below), and Bio-luminescence signal via Luciferase substrate (D-luciferin), In Vivo, and In Vitro.

luciferase and GFP co-expression

Sold at:  1 vial x (2 x 106 cells)/vial, see details in Product Manual.

Cat# SC084-LG