This Pre-made lentiviral particles expresses a point mutation version (G12D) of human target, KRAS (KRAS proto-oncogene, GTPase), Alternative Names: C-K-RAS; c-Ki-ras2; CFC2; K-Ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2. The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_004985.4, except one mutation at amino acid position 12, as G12D.
The target is natively expressed under an optional inducible suCMV promoter. This lentivirus can be used for regular constitutive high expression (without any induction) or used for inducible expression upon tetracycline or Dox induction when the tetracycline repressor protein (TetR) is present.
A RFP_Blasticidin (Fluorescent-Antibiotic) Fusion dual marker under the RSV promoter allows fluorescence sorting or blasticidin resistance selection of transduced cells. The RFP signal additionally provides convenient, real-time monitoring of the lentivirus’ performance.
Each Lentivirus is validated on lot-by-lot basis, and target expression is guaranteed. See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium with 10% FBS and 10x of polybrene (60 ug/ml). (Note: When specifically requested, the concentrated lentivirus can be provided at 1 x 108 IFU/ml in 200ul PBS solution for $650.00 USD).