Premade Luciferase co-express the firefly luciferase 3 reporter and GFP fluorescent reporter, under the same enhanced EF1a promoter. Luciferase and GFP were bicistronically expressed as individual protein, mediated by a 2A element. A Puromycin antibiotic marker was expressed under RSV promoter, which allows to sort or to select transduced cells, via GFP signal or via Puromycin antibiotic (Dual selection). GFP signal provides convenient, realtime monitoring of the lentivirus transduction rate. See Product Manual for details (.pdf).
Lentivirus was concentrated via our proprietary procedures and re-suspended in PBS buffer to obtain the desired ultra-high titer, suitable for all types of in vivo and in vitro applications.
Amount: 50 ul/per vial, at 1 x 109 IFU/ml in PBS (with stablizer).