Pre-made Luciferase (luc) Lentiviral particles expressing firefly luciferase 3 gene under an optional inducible suCMV promoter. A GFP marker was co-expressed under the same promoter. Luciferase and GFP were bicistronically expressed as individual protein mediated by a 2A element. A RFP-Puromycin fusion antibiotic marker (Dual marker) was expressed under RSV promoter. See Product Manual for details (.pdf).
This is double fluorescent labeled luciferase expression lentivirus in which the GFP reflects the luciferase expression (also for monitoring the suCMV promoter activity in your cell line), and the RFP reflects the blasticidin expression (also for monitoring the RSV promoter activity in your cell line). It allows to sort or select transduced cells for long term expression via GFP or RFP signal, as well as via Puromycin antibiotic selection (Dual selection). GFP signal provides a convenient, realtime monitoring the luciferase particles performance.
Particles was concentrated and buffer exchanged into PBS solution.
Amount: 200ul/per vial, at 5 x 107 IFU/ml in PBS.