Lentivirus expresses firefly luciferase 3 gene under an optional inducible suCMV promoter. A RFP marker was co-expressed under the same promoter. Luciferase and RFP were bicistronically expressed as individual protein mediated by a 2A element. A Puromycin antibiotic marker was expressed under RSV promoter, allows to sort or to select transduced cells via RFP signal or via Puromycin antibiotic (Dual selection). RFP signal provides a convenient, realtime monitoring the performance of the particles. See Product Manual for details (.pdf).
Amount: >200ul/per vial, at 1 x 107 IFU/ml in DMEM medium with 10% FBS.