Premade Lentivirus expresses mouse target, CD274 (human CD274 antigen) (alternative name: A530045L16Rik; B7h1; PD-; Pdcd1l; Pdcd1l1; Pdcd1lg1; Pdl1). The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_021893.3.
The target is expressed with C-terminal 6His tag under an optional inducible suCMV promoter. This lentivirus can be used for regular constitutive high expression (without any induction) or used for inducible expression with tetracycline or Dox induction when the tetracycline repressor protein (TetR) is present.
A RFP-Blasticidin fusion dual marker under the RSV promoter allows fluorescence sorting or blasticidin resistance selection of transduced cells. The RFP signal additionally provides convenient, real-time monitoring of the particles’ performance. See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium with 10% FBS and 10x of polybrene (60 ug/ml). (Note: see also the GFP–Puromycin version of this product, CAT#: LVP1418-GP.)