The SEAP (Secreted Embryonic Alkaline Phosphatase) is commonly used as a powerful reporter gene whose signal can be detected via chemiluminescent reporter assay based on dioxetane CSPD (chloro-5-substituted adamantyl-1,2-dioxetane phosphate), and provides a convenient and highly sensitive method for the quantitation of transcriptional activity. SEAP secreted into cell culture supernatant and therefore offers many advantages over intracellular reporters, like luciferase. It allows to determine reporter activity without disturbing the cells, does not require the preparation of cell lysates and can be used for kinetic studies.
This control lentivirus express SEAP reporter under the minimal TATA promoter containing a Null-sequence which do not respond to any signal pathway’s stimulus. It is used to establish the Non-response control profiles to any stimulus while use Pathway Report Lentivirus.
This lentivirus contains the puromycin selection marker under the constitutive RSV promoter. See Product Manual for details (.pdf).
Amount: >200ul/per vial, at 1 x 107 IFU/ml in DMEM medium and 10x Polybrene.