Transformed from Mouse MB49, this cell line co-express the firefly Luciferase 3 and GFP fluorescent report under the enhanced EF1a promoter as two separated proteins (not as fusion), mediated via F2A element. The Puromycin resistance marker was expressed under Rsv promoter. The following express cassette was integrated into cell line’s genome.
Each cell demonstrates strong GFP fluorescent signal and luciferase / chemiluminescence signal via Luciferase assay (Note: It is the pooled polyclonal population, not originated from a single cell colony. Thus you will observe the uneven GFP signal intensity amount cells).
Sold at: 1 vial x (2 x 106 cells)/vial, see details in Product Manual.