Premade lentivirus expresses human target, NT5E (5′-nucleotidase ecto) (alternative name: CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE). The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_002526
The target is expressed with C-terminal 6His tag under an optional inducible suCMV promoter. This lentivirus can be used for regular constitutive high expression (without any induction) or optionally used for inducible expression by tetracycline or Dox induction when the tetracycline repressor protein (TetR) is present.
A RFP–Blasticidin fusion dual marker under the RSV promoter allows fluorescence sorting or blasticidin resistance selection of transduced cells. The RFP signal additionally provides convenient, real-time monitoring of the particles’ performance.
Each Lentivirus is validated on lot-by-lot basis, and target expression is guaranteed. See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium with 10% FBS and 10x of polybrene (60 ug/ml). (Note: When specifically requested, the concentrated particles can be provided at 1 x 108 IFU/ml in 200ul PBS solution for $650.00 USD).