Lentivirus expresses codon optimized Red Fluorescent Protein (RFP) under an enhanced EF1a promoter. A Blasticidin (BSD) selection marker was express under Rsv promoter, which allows to select transduced fluorescent positive cells. The following scheme showed the lentivector core structure:
The re-engineered EF1a promoter is strong constitutive promoter. It is non-tissue specific, highly expressed in all cell types, and less likely be silenced after long-term culture. See Product Manual (.pdf).
- monitor lentivirus transduction efficiency for your cells of interest;
- stable cell line generation;
Amount: 1 x 107 IFU/ml x 200ul in DMEM medium with 10% FBS, 60ug/ml polybrene.