Lentivirus expresses codon optimized Red Fluorescent Protein (RFP) under the enhanced EF1a promoter. It does not contain any antibiotic selection. So if desired, you can only sort the transduced fluorescent positive cells. See Product Manual (.pdf).
- monitor lentivirus transduction efficiency for your cells of interest;
- create RFP expression stable cell line (via fluorescent sorting);
Amount:1 x 107 IFU/ml x 200ul in DMEM medium with 60ug/ml polybrene (10x).