Lentivirus express a fusion target of (RFP-human Histone 2B). The human codon of Histone 2B (H2B) was fusioned, at its N-terminal, with RFP (Red Fluorescent Protein) at its N-terminal, expressed under an enhanced suCMV promoter, containing a Blasticidin antibiotic marker under the RSV promoter.
Fluorescent labeled H2B allowed high-resolution imaging of both mitotic chromosomes and interphase chromatin, and the latter revealed various chromatin condensation states in live cells. It can directly observe the Double Minute Chromosomes (DMs) in living cells without compromising nuclear and chromosomal structures.
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Amount: 200ul/vial (1×107 IFU/ml), provided in DMEM medium with 10% FBS and 60ug/ml Polybrene (10x).