Pre-made Lentivirus expressing a fusion target of (RFP-human Histone 2B). The human Histone 2B (H2B) codon sequence was fusioned, at its N-terminal, with RFP at its N-terminal, expressed under an enhanced suCMV promoter, containing a Puromycin antibiotic marker under the RSV promoter.
Fluorescent labeled H2B allowed high-resolution imaging of both mitotic chromosomes and interphase chromatin, and the latter revealed various chromatin condensation states in live cells. It can directly observe the Double Minute Chromosomes (DMs) in living cells without compromising nuclear and chromosomal structures.
This is the concentrated lentivirus, provided in PBS solution, best for hard-to-transduced cell types.
Please see Product Manual for details.
Amount: 200ul/vial (1×108 IFU/ml), provided in PBS solution with 10% FBS and 60ug/ml Polybrene (10x).