This Lentivirus expresses codon optimized Yellow Fluorescent Protein (RFP) under optional inducible CMV promoter (tetCMV). It carries the Red Fluorescent Protein-Blasticidin (GFP–Puromycin) fusion selection marker under the constitutive RSV promoter, which allows to select transduced cells via antibiotic or fluorescent signal sorting. The following scheme represents its expression cassette:
RFP is constitutively expressed in high level without any induction. But optionally, this particles can be used as Tetracycline inducible expression of RFP in the presence of TetR protein (repressor). The GFP is constitutively expressed and not affected by with or without induction.
It can be used for dual fluorescent protein expression (RFP and GFP) without need for any induction. Also it can used to validate your tetracycline inducible system when TetR is present, with the other fluorescent signal as the internal normalization control. Please see Product manual for details.
Amount: 200ul/per vial, at 1 x 107 IFU/ml in DMEM medium with 10% FBS with 10x polyberne.