Pre-made lentiviral particles expressing a fusion target of (RFP-human KCNN4 ORF). The sequence verified codon sequence of human KCNN4 (NCBI accession ID:NM_002250.2.) was fusioned at its N-terminal with RFP protein, expressed under a consitutive suCMV promoter, containing a puromycin antibiotic marker under the RSV promoter. It can be used to visualize KCNN4’s expressed in cell membrane where the KCNN4 was targeted.
KCNN4 is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx.
Amount: 200ul/vial (1×107 IFU/ml), provided in DMEM medium with 10% FBS and 60ug/ml Polybrene (10x).