This particles was produced in our optional inducible lentiviral system. It expresses codon optimized, Red Fluorescent Protein (RFP) under tetracycline inducible TetCMV promoter. A Puromycin selection under the constitutive RSV promoter, allows to select transduced cells via Puromycin antibiotic. The following scheme showed its expression cassette structure:
RFP is constitutively expressed in high level without any induction. But optionally, RFP can be used as Tetracycline inducible expression in the presence of TetR protein (repressor). Please see Product Manual for details.
RFP signal can be visualized under fluorescent microscope. The Puromycin is constitutively expressed and not affected by Tetracycline induction.
Amount: 200ul/per vial, at 1 x 107 IFU/ml in PBS. The particles was concentrated and buffer exchanged into PBS.