Pre-made lentivirus express reverse tetracycline Transcriptional Activator (rtTA) protein. Lentiviral particles expressing rtTA-m2 gene (ABC65845.1) under an enhanced EF1a promoter (which is non-tissue, non-cell type specific promoter, not be silenced after long-term culture). A RFP-Puromycin fusion dual marker under RSV promoter, allows to sort or select transduced cells for long term expression via RFP signal or via Puromycin antibiotic (Dual markers). See Product Manual (.pdf).
rtTA-M2 is a mutant of rtTA that has increased stability, reduced background expression in the absence of Doxycycline (Dox, tetracycline analog) and improved inducibility in the presence of Dox. It is used for TetOn inducible expression system.
This is the RFP-Puromycin dual marker version of rtTA particles in DMEM medium with 10% FBS with 10% FBS and 10 x Polybrene.
Amount: 200ul/per vial, at 1 x 107 IFU/ml