Premade optional inducible shRNA lentivirus, knockdowning β-Galactosidase (lacZ) gene. Particles contains a RFP-Puromycin dual selection marker. RFP fluorescent signal provide a convenient method to observe the virus transduction under microscope, and the transduced cells can be sorted / selected via either fluorescent signal or Puromycin.
shRNA sequence was designed in β-Galactosidase (lacZ) codon region, demonstrating greater than 90% knockdown levels for exogenous (trasnfected lacZ expression).
Note: Its matched negative control shRNA lentivirus is: CAT# H1(shRNA-Ctr)-RP.
Please see product manual for more details.
Amount: 200ul/tube at (1 x 10 7 IFU/ml) in DMEM medium with 10% FBS, with 10 x polybrene (60 ug/ml),