Premade optional inducible shRNA lentivirus, knockdown firefly luciferase gene. Particles contains a RFP-Puromycin dual selection marker. RFP fluorescent signal provide a convenient method to observe the virus transduction under microscope, and the transduced cells can be sorted / selected via either fluorescent signal or Puromycin.
shRNA sequence was designed in firefly luciferase codon region, demonstrating greater than 75% knockdown levels for exogenous (trasnfected luciferase expression).
Note: Its matched negative control shRNA lentivirus is: CAT# H1(shRNA-Ctr)-RP.
Please see product manual for more details.
Amount: 200ul/tube at (1 x 10 7 IFU/ml) in DMEM medium with 10% FBS, and 10x polybrene (60ug/ml)