Pre-made lentiviral particles express native SV40 large T-antigen gene under engineered suCMV promoter that is most strongest in most cell types. A GFP-Puromycidin fusion dual marker was expressed under RSV promoter which allows to select via Puromycin antibiotic or sort via GFP signal, the transduced cells (Dual markers). GFP signal provides a convenient transduction monitoring for virus performance.
SV40 large T-antigen is widely used in cell immortalization. This lentivirus was concentrated and provided in PBS solution which is best used for serum sensitive culture or for the hard-to-transduce cell types. See Product Manual (.pdf).
Amount: 200ul/per vial at 5 x 107 IFU/ml in PBS