Pre-made lentivirus express SV40 large T-antigen gene under suCMV promoter. A blasticidin–RFP fusion dual marker was expressed under RSV promoter, which allows to select by blasticidin antibiotic or sort via RFP signal, the transduced cells for long term expression (Dual markers). RFP signal provide a convenient transduction monitoring for virus performance.
SV40 large T-antigen can be used for cell immortalization. See Product Manual (.pdf).
Amount: This is the concentrated lentivirus, provided in PBS solution as 200ul/per vial at 1 x 108 IFU/ml