Pre-made lentivirus express SV40 large T-antigen gene under an enhanced EF1a promoter which is active in almost all cell types. It was natively expressed without any tags. A Puromycin resistant gene was expressed under RSV promoter, which allows to select via Puromycin antibiotic, the transduced cells.
SV40 large T-antigen is widely used for cell immortalization. The concentrated lentivirus in PBS is best suitable for hard-to-transduce cell type and for serum sensitive culture. See Product Manual (.pdf).
Amount: This is the concentrated lentivirus, provided in PBS solution as 200ul/per vial at 5 x 107 IFU/ml