Pre-made lentivirus express SV40 large T-antigen gene under an enhanced EF1a promoter. A blasticidin–RFP fusion dual marker was expressed under RSV promoter, which allows to select by blasticidin antibiotic or sort via RFP signal, the transduced cells for long term expression (Dual markers). RFP signal provides a convenient transduction monitoring for virus performance.
SV40 large T-antigen causes cell immortalization and allow to produce high-titer lentivirus in your cell line. It was natively expressed without any tags. See Product Manual (.pdf).
Amount: This is the concentrated lentivirus, provided in PBS solution as 200ul/per vial at 5 x 107 IFU/ml