Pre-made lentiviral particles expresses native SV40 large T-antigen gene under enhanced suCMV promoter. A GFP-Puromycidin fusion dual marker was expressed under RSV promoter, which allows to select via Puromycin antibiotic or sort via GFP signal, the transduced cells (Dual markers). GFP signal provides a convenient transduction monitoring for virus performance.
SV40 large T-antigen is widely used for cell immortalization. See Product manual (.pdf).
Amount: 200ul/per vial at 1 x 107 IFU/ml in DMEM medium with serum and 10x Polbrene.