Pre-made lentiviral particles expressing SV40 large T-antigen gene under suCMV promoter. A RFP-Puromycin fusion dual marker was expressed under RSV promoter, which allows to select, by blasticidin antibiotic or sort via RFP signal, the transduced cells for long term expression. RFP signal provide a convenient transduction monitoring for virus performance.
SV40 large T-antigen causes cell immortalization, and allow to produce high-titer lentivirus in your cell line. It was natively expressed without any tags. See product manual (.pdf).
Amount: 200ul/per vial at 1 x 107 IFU/ml