Description
Lentivirus express MART-1 specific (peptide 1: MMWDRGLGMM) T Cell Receptor (TCR) (Clone DMF5), containing GFP–Puromycin dual selection
The TCR construct express (under the enhanced EF1a promoter), the antibody specifically recognizes the Peptide 1 in antigen MART-1 (Melanoma-associated antigen recognized by T cells-1, or Melan-A). The TCR α chain variable region with a transmembrane (TM) domain and β chain variable region with a transmembrane (TM) domain, are linked by F2A element, as “TCRα(V)/Tm-F2A-TCRβ(V)/Tm”. It also transduces a GFP–Puromycin (fluorescent-antibiotic fusion ) dual selection gene under the separate RSV promoter, which allow to enrich or select the transduced cells via GFP sorting or Puromycin killing. see the Lentivector scheme below
The “target-specific TCRαV and TCRβV sequences” are derived from the verified clones according to published literatures.
About MART-1 (Melanoma Antigen Recognized by T cells 1):
MART-1, also known as Melan-A, is a protein antigen found on the surface of melanocytes. The MART-1 antigen is a target in immunotherapy for patients with melanoma. TCRs targeting MART-1 recognize the antigen on melanoma cells, leading to T cell activation and cytotoxic T cell response to malignant melanoma cells. References: Ref 1, Ref 2.
see details in Product Manual.
Amount: 200 ul/vial at titer of 1×108 IFU/ml, concentrated lentivirus provided in PBS solution, premixed with 10x polybrene (60ug/ml), premade, ready to ship in dry-ice package.
CAT#: LVP1679
