Pre-made inducible lentiviral particles for expressing human target, telomerase reverse transcriptase: TERT (alternative name: EST2, TCS1, TP2, TRT, hEST2), 100% matched CDS region in NCBI ID: NM_198253.2.
The human TERT gene is natively expressed under an optional inducible suCMV promoter. This lentivirus can be used for regular constitutive high expression (without any induction) or used for inducible expression with tetracycline or Dox induction when the tetracycline repressor protein (TetR) is present. [Note: We also provide the consitutive EF1a promoter driven human TERT expression lentivirus with GFP-Puromycin selection marker. Please specify it if you need it under EF1a promoter.]
A GFP–Puromycin fusion dual marker under the RSV promoter allows fluorescence sorting or Puromycin resistance selection of transduced cells. The GFP signal additionally provides convenient, real-time monitoring of the particles’ performance. And, see also the RFP–Blasticidin version of this product, CAT#: LVP105 ]
Each Lentivirus is validated on lot-by-lot basis, and target expression is guaranteed. See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium with 10% FBS and 10x of polybrene (60 ug/ml). (Note: When specifically requested, the concentrated particles can be provided at 1 x 108 IFU/ml in 200ul PBS solution for $650.00 USD).