Pre-made YFP lentivial particles containing codon optimized Yellow fluorescent protein (YFP) marker under suCMV promoter. A Blasticidin antibiotic selection under Rsv promoter allows to select transduced fluorescent positive cells. The following scheme showed the lentivector core structure:
See Product Manual (.pdf).
- monitor lentivirus transduction efficiency for your cells of interest;
- create YFP expression stable cell line (via Blasticidin selection);
Amount:1 x 108 IFU/ml x 200ul in PBS (This is the concentrated virus with higher titer)