Lentivirus express the codon-optimized Yellow fluorescent protein (YFP) under the enhanced EF1a promoter. A Hygromycin antibiotic selection, driven by Rsv promoter, allows to select the transduced fluorescent positive cells. The following scheme showed the lentivector core structure:
See Product Manual (.pdf).
Amount:1 x 107 IFU/ml x 200ul, provided in DMEM, premixed with 10x polybrene (60ug/ml).