Lentivirus expresses the Zika virus’ Envelope Protein. It has the full length of E protein (NCBI ID: AHL43463.1, click to see its Amino Acid sequence), with a poly-histidine tag at the C-terminus (6-His tag) which allow the purification of the antigen via Ni NTA affinity column.
The target was expressed under enhanced CMV promoter. A RFP-Blasticidin fusion dual selection marker under RSV promoter, allows to sort or select transduced cells via RFP fluorescent signal or via Blasticidin antibiotic. RFP signal provides a convenient, realtime monitoring the lentivirus performance. See Product Manual for details (.pdf).
Zika virus (ZIKV) Envelope protein can be used as antigen for virus detection, antibody and vaccines development, and other assays.
Amount: 200ul, at 1 x 108 IFU/ml, concentrated lentivirus, provided in PBS