This Pre-made optional inducible lentiviral particles expresses human target, ZRSR2 (zinc finger CCCH-type, RNA binding motif and serine/arginine rich 2), Alternative Names: U2AF1-RS2; U2AF1L2; U2AF1RS2; URP; ZC3H22. The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_005089
The full sequence-verified target is natively expressed under an optional inducible suCMV promoter. This lentivirus can be used for regular constitutive high expression (without any induction) or used for inducible expression upon tetracycline or Dox induction when the tetracycline repressor protein (TetR) is present.
A GFP–Puromycin fusion dual marker under the RSV promoter allows fluorescence sorting or Puromycin resistance selection of transduced cells. The GFP signal additionally provides convenient, real-time monitoring of the particles’ performance. See Also RFP-Blasticidin version of this product: LVP1118.
Each Lentivirus is validated on lot-by-lot basis, and target expression is guaranteed. See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium with 10% FBS and 10x of polybrene (60 ug/ml). (Note: When specifically requested, the concentrated particles can be provided at 1 x 108 IFU/ml in 200ul PBS solution for $650.00 USD).