Control lentivirus for target over expression and Knockdown shRNA:

GenTarget’s negative control lentiviruses for target overexpression, shRNA knockdown, and optional inducible expression. They serves as the negative controls for lentivirus treatment.

         1.  Target over expression control lentivirus:

The negative control virus for target overexpression lentivirus contains a Null spacer sequence at the target position under an optional inducible CMV promoter (TetCMV), or an enhance CMV promoter or an enhanced EF1a promoter. Due to the lack of a start codon, the Null sequence do not express a target but still contain the selection marker under the RSV promoter. 

Please select the control lentivirus with the same promoter and antibiotic marker as your target expression lentivirus. See the control lentivirus’ core vector scheme below:

The control virus serves as the negative controls for the lentivurs treatment. It validates the specificity of any target expression at the testing cells. 

        2. shRNA expression control lentivirus:

The negative control shRNA lentivirus contains a negative control shRNA sequence under either the optional inducible H1 promoter or constitutive U6 promoter. The Neg-control shRNA lentivirus controls for non-specific knockdown level; it validates the off-target effects of shRNA treatment. See control vector map scheme below.

The negative expression control lentiviruses contain an antibiotic selection marker corresponding to each shRNA expression lentivector. Please select the control lentivirus with the same promoter and antibiotic marker as your shRNA expression lentivirus. See each shRNA control virus product link for details.

        3. Optional inducible expression control lentivirus:

GenTarget provides pre-made control viruses for optional inducible expression , used for controls in the a specific human, mouse or rat’s gene over-expression lentivirus products, or used to validate the optional inducible system in your specific cell lines.  (See vector scheme above).

The inducible controls contain GFP or RFP under control of the inducible promoter, enabling very convenient verification of tetracycline inducible effects by simple monitoring of GFP or RFP signal under a microscope. The controls also contain a fluorescent-antibiotic fusion dual marker under a RSV promoter which is not affected by induction. The dual marker provides a convenient way to monitor viral transduction efficiency and select positive cells by either antibiotic killing or fluorescence sorting. see the core lentivector scheme below: