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Home / Stable cell lines / rtTA stable cells

rtTA stable cells

rtTA (reverse tetracycline transcriptional activator) expression cell lines:

Pre-made stable cell line, derived from Hela cells, constitutively expresses the rtTA-m2 (ABC65845.1) different fluorescent-antibiotic fusion markers.

The rtTA-m2 was expressed in high level under the enhanced EF1a promoter. The antibiotic marker or the fluorescent-antibiotic marker is constitutively expressed under the RSV promoter. Please see the  integrated expression cassette (below).

rtTA stable cell map

Application for rtTA Expression Cell Lines:

This cell lines express the rtTA protein which can bind to tetracycline operator elements (TetO) in the presence of doxycycline (Dox). Any promoters that embedded with TetO sequences can binds to the rtTA protein when the inducer (Dox or tetracycline) is present, which activates the transcription of the promoter’s downstream gene (So called, Tet-On inducible system). Therefore, this cell line can be used for Tetracycline or Dox inducible target expression when applied with target expression vectors (driven by TetO inducible promoter), or for generation of any inducible target stable cell lines (after introduced a TetOn expression vector).

Note:  GenTarget provide stable cell line services. Please Contact Us or Email us for a service quote.

Please click each product link below for product details, or see Product Manual.

NameSKUPriceBuy
rtTA (GFP-Bsd) / Hela stable cellsSC038-GB$1,100.00
rtTA (GFP-Puro) / Hela stable cellsSC038-GP$1,100.00
rtTA (RFP-Bsd) / Hela stable cellsSC038-RB$1,100.00

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    • Cell-Specific Reporter
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    • Virus Like Particles (VLP)
    • Non-integrating LV
    • shRNA lentivirus
    • microRNA lentivirus
    • anti miRNA lentivirus
    • Lentivectors
    • Control lentivirus
    • Ultra titer lentivirus
    • Eco PCR Cloning Kits
    • Reagents for Lentivirus
  • Services
    • Lentivirus Service
    • Customized Stable Cell Lines
    • Fusion In Vivo PCR Cloning
    • Protein Expression
    • Antibody Cloning and Expression
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    • Protocols
    • MSDS Files
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