Description
Lentivirus express human codon-optimized AsCas12a_mutant (click to see its Amino-acid sequence) under suCMV promoter, with a NLS (Nuclear Localization Signal) for the best nuclear penetration, and FLAG tag which provides a convenient tool to detect or purify the expressed AsCas12a enzyme. It also contains a Hygromycin antibiotic marker for the selection of the transduced cells. See Product Manual for details (.pdf).
The Following mutations were engineered to enhances CRISPR gene editing success rate.
Application: used for CRISPR gene editing; purification of Cas12a enzyme; generate Cas12a expression stable cell lines.
Amount: 1×10^8 IFU/mlx 200ul (provided in PBS solution, premixed with 10x Polybrene, 60ug/ml)
Shipping and storage: overnight shipment in dry-ice package. Stored at -80oC.
Cat#: LVP1822
