Hygromycin B is produced by Streptomyces hygroscopicus. It inhibits polypeptide synthesis by binding to the 80S subunit, therefore, inhibiting translation. Resistance to HYGROMYCIN B is conferred by a kinase that inactivates hygromycin B through phosphorylation.
It is used in cell biology as a selective agent to select transformed cells which have been engineered to the hygromycin-resistance gene.
It is toxic to prokaryotic and eukaryotic cells, thus used as a selection maker for Hygromycin-resistant transforms in mammalian cells, plant cells, or bacteria at its working concentration.
The working concentration of Gentarget’s Hygromycin solution were tested in many mammalian cell types against Gentarget’s lentivirus products containing hygromycin resistance, see details in Product Manual (pdf)
Amount: 1.0 ml/tube at 50 mg/mL.