Cas9 (CMV, RFP-Bsd) Expression Lentivirus, Concentrated

$650.00

SKU: LVP707-PBS Categories: ,

Description

 

Cat#: LVP707-PB

Lentivirus express human codon-optimized Cas9 endonuclease (Streptococcus pyogenes )  under suCMV promoter, with a proprietary NLS (Nuclear Localization Signal) at its N-terminal, for the best nuclear penetration. It also contains a RFP-Blasticidin fusion  Fluorescent-antibiotic dual marker for the selection of the transduced cells. (see also the Non-Integration Lentivirus of this product, CAT#: IDLV010A.)

This is the concentrated lentivirus with higher titer. See Product Manual here (.pdf).

Application: used for CRISPR gene editing ( Note: used with a separate “gene-specific gRNA lentivirus” or ” gene-specific gRNA vector” for CRISPR editing )

Amount:  5 x 107 IFU/ml x 200ul in DMEM medium with 10% FBS, 10x of polybrene. 

Shipping and storage: overnight shipment in dry-ice package. Stored at -80oC.

CAT#: LVP707-PBS