Pre-made lentivirus express the tetracycline regulator gene under an enhanced CMV promoter. A GFP-Blasticidin fusion dual marker, driven by RSV promoter, allows to sort or select transduced cells via GFP signal or via blasticidin antibiotic (Dual markers). See Product Manual (.pdf).
TetR is used in tetracycline-inducible expression. It binds to any inducible promoters that have incorporated its binding sequence to repress target expression. And target expression is induced once tetracycline is added. Tetracycline binds to TetR, which releases TetR from target’s promoter, and starts the transcription. Gentarget’s Optional inducible shRNA Particles and Optional inducible expression particles can be turned into tetracycline-inducible system by using TetR expressing particles. By using a different antibiotic marker in TetR particles than that in expression particles, you can select the double transduced cells via double antibiotic selection at the same time. Then, the target’s expression is turned into a tetracycline dose-dependent inducible expression.
This is the concentrated lentivirus in PBS with GFP-Blasticidin dual maker (see also the EF1a promoter version of this product, CAT#: CAT#: LVP459-GB-PBS)
Amount: 200ul/per vial, at 1 x 107 IFU/ml.