Pre-made tetracycline regulator (TetR) lentiviral particles expressing the tetracycline regulator gene under suCMV promoter (providing the highest TetR expression level). A Hygromycin antibiotic marker under RSV promoter, allows to select transduced cells for long term expression. See Product Manual (.pdf).
TetR is used in tetracycline inducible expression. It binds to any inducible promoters that has incorporated its binding sequence to repress target expression. And target expression is induced once tetracycline is added. Tetracycline binds to TetR, which releases TetR from target’s promoter, and starts the transcription. Gentarget’s Optional inducible shRNA particles and Optional inducible expression particles can be turned into tetracycline inducible system by using TetR expressing particles. By using a different antibiotic marker in TetR particles than that in expression particles, you can select the double transduced cells via double antibiotic selection at the same time. Then, the target’s expression is turned into a tetracycline dose dependent inducible expression.
This is the Hygromycin version of TetR particles purified into PBS solution.
Amount: 200ul/per vial, at 1 x 108 IFU/ml in PBS.