COVID-19 S (6His) / (Null) VLP

$650.00

SKU: VLP003 Category:

Description

COVID-19 Spike protein (S) Virus Like Particle was packaged in HEK293T cells using our proprietary technology. It is the replication-incompetent particles in which the native, full-length COVID-19 S protein (with 6His-tag at its C-terminal) was pseudo-typed as the lentiviral particles’ surface envelope protein.  [Note: This VLP has the Spike protein already expressed / presented at surface of lentiviral particle to mimic coronavirus. It is not used as lentivirus for over-expression of the Spike protein. For over-expressing COVID-19 S protein, please use lentivirus product, CAT#: LVP1329.]

This VLP packaged a non-sense sequence (Null) as the particle’s virion genomic material.  When the COVID-19 S protein binding receptor (such as ACE2) is present on the cell surface, this VLP will bind onto the cells.

Applications:

COVID-19 S Protein VLP has been tested in response to the binding of anti-S antibody in ELISA plate. The S protein was assembled as surface antigens for use in the development of COVID-19 diagnostics and in vaccine development and other validation assays for COVID-19 coronavirus. This VLP can be detected by anti-C-terminal 6His-tag antibody because the S protein was tagged by C-term 6His-tag.

The VLP’s titer was measured via ELISA P24 assay. Every single particle assembled many S protein molecular at its surface. See more details in Product Manual.

Amount: 200ul/vial at 1×108 VP/ml.

CAT#: VLP003

Reference

Front Bioeng. Biotechnol. 30 July 2020; Ruodan Xu et al. Construction of SARS-CoV-2 Virus-Like Particles..