Lentivirus expresses the receptor binding domain (RBD) of S1 protein in COVID-19 (SARS-CoV-2) coronavirus. It has the human codon optimization for high expression in mammalian cells. It is expressed under a secretion leader. The secreted expressed S1-RBD (click to see its Amino Acid sequence) is about ~45 kDa, with a poly-histidine tag at the C-terminus (6-His tag) which allow to purify the S1-RBD Protein directly from the culture medium without need for cell lysis, via Ni NTA affinity column, .
The target was expressed under enhanced CMV promoter. A RFP-Blasticidin fusion dual selection marker under RSV promoter, allows to sort or select transduced cells via RFP fluorescent signal or via Blasticidin antibiotic. RFP signal provides a convenient, realtime monitoring the lentivirus performance. See Product Manual for details (.pdf).
Amount: 200ul, at 1 x 107 IFU/ml in DMEM medium with 10x of polybrene (60 ug/ml). (Note: When specifically requested, the concentrated particles can be provided at 1 x 108 IFU/ml in 200ul PBS solution for $650.00 USD). (see also product, CAT#: LVP1329).