Pre-made micro RNA exrepssion lentivirus: Human microRNA precursors and its native context sequences (upstream and downstream flanking genomic sequences) were PCR amplified and cloned into a pLenti-TetCMV(GFP-3UTR/miRNA)-Rsv(Puro) lentivector. See Product Manual for details.
1) 500 ul of ready-to-use specific miRNA expression lentivirus in DMEM-medium containing 60 ug polybrene (10x).
2) 500 ul of ready-to-use Negative-control miRNA expression lentivirus in DMEM-medium containing 60 ug polybrene (10x). (Note: The miR-Control express the GFP marker, but not any miRNA sequence).
Virus titer: 1×107 IFU/ml,
Shipping: overnight shipment in dry-ice package. (Note: this is made-on-demand order, the timeline is 2 weeks to delivery)
CAT#: H-GFP-miR (Note: please provide the microRNA ID or microRNA express catalog # at check out.)