Lentivirus expresses tetracycline regulator gene (click to see sequence) under an enhanced EF1a promoter (which is a non-tissue, non-cell type-specific promoter, not be silenced after long-term culture). A Blasticidin antibiotic selection was expressed under RSV promoter, allowing to select transduced cells. See Product Manual (.pdf).
TetR is used in tetracycline-inducible expression. It binds to any inducible promoters that has incorporated its binding sequence to repress target expression. And target expression is induced once tetracycline is added. Tetracycline binds to TetR, which releases TetR from target’s promoter, and starts the transcription. Gentarget’s lentiviral inducible shRNA vectors and inducible expression particles can be turned into tetracycline inducible system by using TetR expressing particles. By using a different antibiotic marker in TetR particles than that in expression particles, you can select the double transduced cells via double antibiotic selection at the same time. Then, the target’s expression is turned into a tetracycline dose-dependent inducible expression.
This is the blasticidin version of TetR particles in DMEM medium with 10% FBS.
Amount: 200ul/per vial, at 1 x 107 IFU/ml.