h CD274 / PDL1 (EF1a, Bsd), Concentrated Lentivirus


SKU: LVP1502-PBS Categories: ,


Lentivirus expresses human target, CD274 / PDL1 (human CD274 molecule) (alternative name: B7-H; B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1). The sub-cloned codon sequence is identical (100% match) to CDS region  in NCBI ID: NM_014143.

The target is expressed with C-terminal 6His tag under an enhanced EF1a promoter which is best for suspension cells, like T cells, or B cells (or so called Hard-to-transduced cell type or cell types have weak CMV promoter strength).  The lentivirus contains a Blasticidin selection under the RSV promoter.  See the expression cassette scheme below:

target expression scheme under EF1a promoter

Amount: 200ul, at 1 x 108 IFU/ml , provided in PBS solution [premixed with 10x of polybrene (60 ug/ml].  (Note: see also the RFP-Blasticidin dual selection version of this product, driven by CMV promoter, CAT#: LVP1077

Cat#: LVP1502-PBS